Mean (SD) change from baseline in cytokine values, and absolute lymphocyte counts with time in patients with RA treated with methotrexate (n=17)
| Months in study | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cytokine (pg/mL) | Baseline | 6 | 7 | 8 | 12 | 18 | 24 | 30 | 36 | |
| IL-1BPt | 188.2 | (173.2) | 130 | −94 | 12 | 92 | 15 | 102 | 160 | 225 |
| (353) | (165) | (134) | (174) | (13) | (102) | (98) | (178) | |||
| IL-1BSH | 72.2 | (94.2) | −34+ | −41** | −29** | −19 | −39* | −26* | −32 | −72 |
| (51) | (50) | (33) | (48) | (33) | (40) | (49) | (94) | |||
| IL-2P | 300.0 | (197.5) | 183 | 330* | 193 | 503** | 285* | 540* | 289** | 398* |
| (314) | (424) | (352) | (442) | (268) | (572) | (232) | (388) | |||
| IL-2S | 55.3 | (11.4) | 32 | 12 | 16 | 7 | 22 | 12 | −9 | −31 |
| (23) | (23) | (32) | (60) | (32) | (6) | (65) | (35) | |||
| IL-4P | 26.7 | (36.9) | −9 | −4 | 10 | 9* | 11 | 21 | 6 | 17 |
| (23) | (51) | (35) | (9) | (5) | (21) | (7) | (10) | |||
| IL-4S | 6.2 | (8.2) | −4 | −4 | −12 | 2 | −5 | −7 | −0.4 | – |
| (14) | (11) | (12) | (3) | (11) | (12) | (2) | ||||
| IL-6S | 44.1 | (25.3) | −33+ | −28** | −28* | −25** | −37** | −37+ | −46+ | −2.8 |
| (21) | (27) | (35) | (27) | (25) | (26) | (25) | (32) | |||
| IL-8S | 457.4 | (722.1) | −424* | −302 | −419 | −481 | −826* | −411* | −344 | −111 |
| (663) | (736) | (669) | (881) | (760) | (642) | (627) | (487) | |||
| Baseline | 6 | 7 | 8 | 12 | 24 | 30 | 36 | ||
|---|---|---|---|---|---|---|---|---|---|
| CD4 | 645 | (519) | 310 | 149 | −35 | −25 | −136 | 144 | – |
| (446) | (798) | (443) | (493) | (581) | (687) | ||||
| CD8 | 282 | (182) | −23 | 12 | −10 | −69 | 18 | −26 | – |
| (140) | (137) | (186) | (186) | (156) | (109) | ||||
| CD4/CD8 | 255 | (485) | −183 | −75 | 69 | −203 | −26 | −274 | – |
| (335) | (187) | (135) | (795) | (81) | (410) | ||||
| CD4/CD29 | 426 | (288) | −16 | 23 | −6.3 | −170 | −16 | −194++ | – |
| (111) | (214) | (187) | (322) | (261) | (102) | ||||
| CD4/CD45RA | 531 | (315) | −44 | −207 | 84 | 58 | −58 | −110 | – |
| (255) | (352) | (319) | (221) | (395) | (253) | ||||
| CD5/CD19 | 99 | (173) | −10 | – | −110 | 982 | −7.5 | −73 | – |
| (42) | (114) | (–) | (13) | (114) |
t supernatants from peripheral blood mononuclear cells.
H S=serum.
*p<0.05.
**p<0.01.
***Values obtained by multiplying total absolute lymphocyte counts by the percentage of each subpopulation as measured by FACS analysis (see the Methods section).
+p<0.001.
++p<0.05.
IL, interleukin; RA, rheumatoid arthritis.