Summary
Immunohistochemical localization of types I, II, and III collagen in the ossified posterior longitudinal ligament of the cervical spine was studied using type-specific anticollagen antibodies. In contrast to the normal ligament which contains both types I and III collagens, the ossified matrix, composed of lamellar bone, contains only type I collagen, except for Haversian canals where type III is located in the inner wall. In the transitional region of preossifying ligaments, types III and I are both present. Type II collagen is present in the hyperplastic matrix of the ligament, and cartilage-like cells surrounded by type II collagen are aligned along nonossified ligaments adjacent to the preossifying region. A possible mechanism of matrix transition during the ossification process is given attention.
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References
Ono K, Ota H, Tada K, Hamada H, Takaoka K (1977) Ossified posterior longitudinal ligament: A clinico-pathologic study. Spine 2:126–138
Investigation Committee on OPLL of the Ministry of Public Health and Welfare of Japan (1981) The ossification of the posterior longitudinal ligament of the spine. J Jpn Orthop Ass 55:425–440
Gay S, Miller EJ (1978) Collagen in the physiology and pathology of connective tissue. Gustav Fischer Verlag, Stuttgart, New York
Sano J, Fujiwara S, Sato S, Ishizaki M, Sugisaki Y, Yajima G, Nagai Y (1981) AB (type V) and basement membrane (type IV) collagens in the bovine lung parenchyma: Electron microscopic localization by the peroxidase-labeled antibody method. Biomedical Res 2:20–29
Konomi H, Hori H, Sano J, Sunada H, Hata R, Fujiwara S, Nagai Y (1981) Immunohistochemical localization of type I, II, III, and IV collagens in the lung. Acta Pathol Jpn 31:601–610
von der Mark H, von der Mark K, Gay S (1976) Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. I. Preparation of collagen type I and type II specific antibodies and their application to early stages of the chick embryo. Dev Biol 48:237–249
von der Mark K, von der Mark H (1977) The role of three genetically distinct collagen types in endochondral ossification and calcification of cartilage. J Bone Joint Surg 59-B:458–464
Linsenmayer TF, Toole BP, Trelstad RL (1973) Temporal and spatial transition in collagen types during embryonic chick limb development. Dev Biol 35:232–239
Reddi AH, Gay R, Gay S, Miller EJ (1977) Transition in collagen types during matrix-induced cartilage, bone, and bone marrow formation. Proc Nat Acad Sci USA 74:5589–5592
Hayashi T, Nakamura T, Hori H, Nagai Y (1980) Degradation rates of type II and III collagens by tadpole collagenase are modulated by mutual presence. J Biochem 87:993–995
Yasui N, Hori H, Nagai Y (1981) Production of collagenase inhibitor by the growth cartilage of embryonic chick bone: Isolation and partial characterization. Coll Rel Res 1:59–72
Sunada H, Hayashi T, Hori H, Nagai Y (1981) Dual effects of type II collagen on the degradation of type I collagen by tadpole collagenase. Coll Rel Res 1:177–185
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Yasui, N., Ono, K., Yamaura, I. et al. Immunohistochemical localization of types I, II, and III collagens in the ossified posterior longitudinal ligament of the human cervical spine. Calcif Tissue Int 35, 159–163 (1983). https://doi.org/10.1007/BF02405024
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DOI: https://doi.org/10.1007/BF02405024