Abstract
Several cell surface markers were used to isolate monocytes as osteoclast progenitors with an immunomagnetic cell separation system. Use of this system with specific monocyte antibodies produced 99% pure monocytes. When purified monocytes were cultured on bovine bone slices in the presence of receptor activator of nuclear factor-κB (RANKL), macrophage-colony stimulating factor (M-CSF), tumor necrocis factor alpha (TNF-α), and dexamethasone for 14 days, CD14+ CD11b+, and CD61+ monocytes had approximately 90-, 30- and 20-fold higher osteoclast formation capacities/plated cells compared to the control culture. CD15+ monocytes generated few tartrate-resistant acid phosphatase-positive multinucleated cells (TRACP+ MNC), and CD169+ monocytes generated no TRACP+ MNC. This suggests, that there are various subsets of monocytes in the blood circulation and that they have different capacities in osteoclast formation. These results show that circulating human osteoclast progenitors can be efficiently purified by immunomagnetic cell separation system using anti-CD14, -CD11b, and -CD61 antibodies. These purified monocyte fractions had different ability to give rise to osteoclasts. CD169 was not found to be suitable for osteoclast progenitor isolation. Optimal concentration of dexamethasone for osteoclast formation and bone resorption was 10 nM. To develop a human resorption assay, osteoclasts were first induced for 7 days, whole media were replaced, cultures were continued for additional 3 days and C-terminal telopeptide of type I collagen was determined from culture media. This assay was shown to be functional, since two well-known resorption inhibitors, bafilomycin A1 and calcitonin, dose-dependently inhibited the resorption activity of osteoclasts.
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References
BA Scheven JW Visser PJ Nijweide (1986) ArticleTitleIn vitro osteoclast generation from different marrow fractions including a highly enriched haematopoitic stem cell population Nature 321 79–81 Occurrence Handle1:STN:280:BimB3czmsFE%3D Occurrence Handle3754620
CE Hagenaars AA Kraan Particlevan der EW Kawilarang-de Haas JW Visser PJ Nijweide (1989) ArticleTitleOsteoclast formation from cloned pluripotent hematopoietic stem cell Bone Miner 6 179–189 Occurrence Handle10.1016/0169-6009(89)90049-4 Occurrence Handle1:STN:280:BiaA2M3pvVE%3D Occurrence Handle2788471
G Hattersley TJ Chambers (1989) ArticleTitleGeneration of osteoclasts from hematopoietic cell and multipotential cell in vitro J Cell Physiol 140 478–482 Occurrence Handle10.1002/jcp.1041400311 Occurrence Handle1:CAS:528:DyaL1MXlvFais7o%3D Occurrence Handle2777887
T Suda N Takahashi TJ Martin (1992) ArticleTitleModulation of osteoclast differentiation Endoc Rev 13 66–80 Occurrence Handle10.1210/er.13.1.66 Occurrence Handle1:STN:280:By2B3cbksVE%3D
A Matayoshi C Brown JF DiPersio J Haug Y Abu-Amer H Liapis R Kuestner R Pacifici (1996) ArticleTitleHuman blood mobilized hematopoitic precursors differentiate into osteoclasts in the absence of stromal cell Proc Natl Acad Sci USA 93 10785–10790 Occurrence Handle10.1073/pnas.93.20.10785 Occurrence Handle1:CAS:528:DyaK28XmtVOltb0%3D Occurrence Handle8855258
DG Walker (1973) ArticleTitleOsteoporosis cured by temporary parabiosis Science 180 875 Occurrence Handle1:STN:280:CSyC1czmvFw%3D Occurrence Handle4706681
AJ Kahn DJ Simmons (1975) ArticleTitleInvestigation of the cell lineage in bone using a chimera of chick and quail embryonic tissue Nature 258 325–327 Occurrence Handle1:STN:280:CSmD2srmvVE%3D Occurrence Handle1196357
DJ Walker (1975) ArticleTitleControl of bone resorption by hematopoietic tissue. The induction and reversal of congenital osteopetrosis in mice through the use of bone marrow mononuclear phagocytes J Exp Med 142 651–663 Occurrence Handle10.1084/jem.142.3.651 Occurrence Handle1:STN:280:CSmD38njslM%3D Occurrence Handle1100763
PF Coccia W Krivit J Cervenka C Clawson JH Kersey TH Kim ME Nesbit NK Ramsay PI Warkentin SL Teitelbaum AJ Kahn DM Brown (1980) ArticleTitleSuccessful bone marrow transplantation for infantile malignant osteopetrosis N Engl J Med 302 701–708 Occurrence Handle1:STN:280:Bi%2BC38%2FlsFY%3D Occurrence Handle6986555
J Faust DL Lacey P Hunt TL Burgess S Scully A Eli Y Qian V Shalhoub (1999) ArticleTitleOsteoclast markers accumulate on cells developing from human peripheral blood mononuclear precursors J Cell Biochem 72 67–80 Occurrence Handle10.1002/(SICI)1097-4644(19990101)72:1<67::AID-JCB8>3.0.CO;2-A Occurrence Handle1:CAS:528:DyaK1cXnvVegsrw%3D Occurrence Handle10025668
TJ Chambers (1980) ArticleTitleThe cellular basis of bone resorption Clin Orthop 151 283–293 Occurrence Handle7418318
GD Roodman (1991) ArticleTitleOsteoclast differentiation Crit Rev Oral Biol Med 2 389–409 Occurrence Handle1:STN:280:By6A2s%2FitVQ%3D Occurrence Handle1892993
Y Fujikawa JM Quinn A Sabokbar JO McGee NA Athanasou (1996) ArticleTitleThe human osteoclast precursor circulates in the monocyte fraction Endocrinology 137 4658–4661 Occurrence Handle10.1210/en.137.9.4058
EH Burger JW Meer ParticleVan der JS Gevel JC Gribnau GW Thesingh R Furth Particlevan (1982) ArticleTitleIn vitro formation of osteoclasts from long-term cultures of bone marrow mononuclear phagocytes J Exp Med 156 1604–1614 Occurrence Handle10.1084/jem.156.6.1604 Occurrence Handle1:STN:280:BiyD1cvlslQ%3D Occurrence Handle7175438
GB Schneider M Relfson (1988) ArticleTitleA bone marrow fraction enriched for granulocyte-macrophage progenitors gives rise to osteoclasts in vitro Bone 9 303–308 Occurrence Handle10.1016/8756-3282(88)90014-2 Occurrence Handle1:STN:280:BiaD2snms1Q%3D Occurrence Handle3203017
N Kurihara S Gluck GD Roodman (1990) ArticleTitleSequential expression of phenotype markers for osteoclasts during differentiation of precursors for multinucleated cells formed in long-term human marrow cultures Endocrinology 127 3215–3221 Occurrence Handle1:CAS:528:DyaK3MXktVSgug%3D%3D Occurrence Handle1701138
G Mbalaviele P Orcel C Morieux PJ Nijweide MC Vernejoul Particlede (1995) ArticleTitleOsteoclast formation from human cord blood mononuclear cells co-cultured with mice embryonic metatarsals in the presence of M-CSF Bone 16 171–177 Occurrence Handle10.1016/S8756-3282(94)00026-3 Occurrence Handle1:CAS:528:DyaK2MXkt1eqtbo%3D Occurrence Handle7742078
G Cournot M Petrovic CL Trubert C Cormier D Girault A Fischer M Garabedian (1993) ArticleTitleCultured circulating mononuclear cells from osteopetrotic infants express the osteoclast-associated vitronectin receptor and form multinucleated cells in response to 1,25-dihydroxyvitamin D3 J Bone Miner Res 8 61–70 Occurrence Handle1:STN:280:ByyC2M%2FpsFw%3D Occurrence Handle7678950
T Suda N Takahashi N Udagawa E Jimi MT Gillespie TJ Martin (1999) ArticleTitleModulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families Endocrine Rev 20 345–357 Occurrence Handle10.1210/er.20.3.345 Occurrence Handle1:CAS:528:DyaK1MXktVaqtLg%3D
YY Kong H Yoshida I Sarosi HL Tan E Timms C Capparelli S Morony AJ Oliveira-dos-Santos G Van A Itie W Khoo A Wakeham CR Dunstan DL Lacey TW Mak WJ Boyle JM Penninger (1999) ArticleTitleOPGL is a key regulator of osteoclastogeneis, lymphocyte development and lymph-node organogenesis Nature 397 315–323 Occurrence Handle10.1038/16852 Occurrence Handle1:CAS:528:DyaK1MXhtVOqs7w%3D Occurrence Handle9950424
H Yasuda N Shima N Nakagawa K Yamaguchi M Kinosaki S Mochizuki A Tomoyasu K Yano M Goto A Murakami E Tsuda T Morinaga K Higashio N Udagawa N Takahashi T Suda (1998) ArticleTitleOsteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL Proc Natl Acad Sci USA 95 3697–3602 Occurrence Handle10.1073/pnas.95.7.3597
DL Lacey E Timms HL Tan MJ Kelley CR Dunstan T Burgess R Elliott A Colombero G Elliott S Scully H Hsu J Sullivan N Hawkins E Davy C Capparelli A Eli YX Qian S Kaufman I Sarosi V Shalhoub G Senaldi J Guo J Delaney WJ Boyle (1998) ArticleTitleOsteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation Cell 93 165–176 Occurrence Handle10.1016/S0092-8674(00)81569-X Occurrence Handle1:CAS:528:DyaK1cXivVyhtrg%3D Occurrence Handle9568710
N Nakagawa M Kinosaki K Yamaguchi N Shima H Yasuda K Yano T Morinaga K Higashio (1998) ArticleTitleRANK is essential signaling receptor for osteoclast differentiation factor in osteoclastogenesis Biochem Biophys Res Commun 253 395–400 Occurrence Handle10.1006/bbrc.1998.9788 Occurrence Handle1:CAS:528:DyaK1MXhs12ntw%3D%3D Occurrence Handle9878548
K Matsuzaki N Udagawa N Takahashi K Yamaguchi H Yasuda N Shima T Morinaga Y Toyama Y Yabe K Higashio T Suda (1998) ArticleTitleOsteoclast differentiation factor (ODF) induces osteoclast-like cell formation in human peripheral blood mononuclear cells culture Biochem Biophys Res Commun 246 199–204 Occurrence Handle10.1006/bbrc.1998.8586 Occurrence Handle1:CAS:528:DyaK1cXjtFWru7w%3D Occurrence Handle9600092
ER Stanley LJ Guilbert RJ Tushinski SH Bartelmez (1983) ArticleTitleCSF-1-a mononuclear phagocyte lineage-specific hemopoieric growth factor J Cell Biochem 21 151–159 Occurrence Handle10.1002/jcb.240210206 Occurrence Handle1:STN:280:BiyB1M7jsVU%3D Occurrence Handle6309875
S Tanaka N Takahashi N Udagawa T Tamura T Akatsu ER Stanley T Kurokawa T Suda (1993) ArticleTitleMacrophage colony-stimulating factor is indispensable for both proliferation and differentiation of osteoclast progenitors J Clin Invest 91 257–263 Occurrence Handle1:CAS:528:DyaK3sXhs1WltLs%3D Occurrence Handle8423223
S Yang Y Zhang RM Rodriguiz WL Ries LL Key SuffixJr (1996) ArticleTitleFunctions of the M-CSF receptor on osteoclasts Bone 18 355–360 Occurrence Handle10.1016/8756-3282(96)00013-0 Occurrence Handle1:STN:280:BymB1M7ks1Q%3D Occurrence Handle8726394
K Selander P Lehenkari HK Vaananen (1994) ArticleTitleThe effects of bisphosphonates on the resorption cycle of isolated osteoclasts Calcif Tissue Int 55 368–375 Occurrence Handle10.1007/BF00299317 Occurrence Handle1:CAS:528:DyaK2MXhvFWhtLw%3D Occurrence Handle7532540
Y Fujikawa JM Quinn A Sabokbar JO McGee NA Athanasou (1996) ArticleTitleThe human osteoclast precursor circulates in the monocyte fraction Endocrinology 137 4658–4661 Occurrence Handle10.1210/en.137.9.4058
JM Quinn S Neale Y Fujikawa JO McGee NA Athanasou (1998) ArticleTitleHuman osteoclast formation from blood monocytes, peritoneal macrophages and bone marrow cells Calcif Tissue Int 62 527–531 Occurrence Handle10.1007/s002239900473 Occurrence Handle1:CAS:528:DyaK1cXjtlKqsL8%3D Occurrence Handle9576981
V Shalhoub J Faust WJ Boyle CR Dunstan M Kelley S Kaufman S Scully G Van DL Lacey (1999) ArticleTitleOsteoprotegerin and osteoprotegerin ligand effects on osteoclast formation from human peripheral blood mononuclear cell precursors J Cell Biochem 72 251–261 Occurrence Handle10.1002/(SICI)1097-4644(19990201)72:2<251::AID-JCB9>3.0.CO;2-W Occurrence Handle1:CAS:528:DyaK1MXjt1yqsw%3D%3D Occurrence Handle10022507
GC Nicholson M Malakellis FM Collier PU Cameron WR Holloway TJ Gough C Gregorio-King MA Kirkland DE Myers (2000) ArticleTitleInduction of osteoclasts from CD14-positive human peripheral blood mononuclear cells by receptor activator of nuclear factor kappaB ligand (RANKL) Clin Sci 99 133–140 Occurrence Handle10.1042/CS19990355 Occurrence Handle1:CAS:528:DC%2BD3cXmsV2murc%3D Occurrence Handle10918046
Y Azuma K Kaji R Katogi S Takeshita A Kudo (2000) ArticleTitleTumor necrosis factor-a induces differentiation and bone resorption by osteoclasts J Biol Chem 275 4858–4864 Occurrence Handle10.1074/jbc.275.7.4858 Occurrence Handle1:CAS:528:DC%2BD3cXhsVyms74%3D Occurrence Handle10671521
J Lam S Takeshita JE Barker O Kanagawa FP Ross SL Teitelbaum (2000) ArticleTitleTNF-alpha induces osteoclastogenesis by direct stimulation of macrophages exposed to permissive levels of RANK ligand J Clin Invest 106 1481–1488 Occurrence Handle1:CAS:528:DC%2BD3cXovFagtbo%3D Occurrence Handle11120755
E Jimi T Shuto T Koga (1995) ArticleTitleMacrophage colony-stimulating factor and interleukin-1a maintain the survival of osteoclast-like cells Endocrinology 136 808–811 Occurrence Handle10.1210/en.136.2.808 Occurrence Handle1:CAS:528:DyaK2MXjtleiu7c%3D Occurrence Handle7835314
U Sarma AM Flanagan (1996) ArticleTitleMacrophage-stimulating factor induces substantial osteoclast generation and bone resorption in human bone marrow cultures Blood 88 2531–2540 Occurrence Handle1:CAS:528:DyaK28XmtFSju78%3D Occurrence Handle8839845
T Hirayama A Sabokbar NA Athanasou (2002) ArticleTitleEffect of corticosteroids on human osteoclast formation and activity J Endocrinol 175 155–163 Occurrence Handle10.1677/joe.0.1750155 Occurrence Handle1:CAS:528:DC%2BD38XosVKjsro%3D Occurrence Handle12379499
K Kobayashi N Takahashi E Jimi N Udagawa M Takami S Kotake N Nakagawa M Kinosaki K Yamaguchi N Shima H Yasuda T Morinaga K Higashio TJ Martin T Suda (2000) ArticleTitleTumor necrosis factor alpha stimulates osteoclast differentiation by a mechanism independent of ODF/RANKL-RANK-interaction J Exp Med 191 257–286 Occurrence Handle10.1084/jem.191.2.275
V Shalhoub G Elliott L Chiu R Manoukian M Kelley N Hawkins E Davy G Shimamoto J Beck SA Kaufman S Scully M Qi M Grisanti C Dunstan WJ Boyle DL Lacey (2000) ArticleTitleCharacterization of osteoclast precursors in human blood Br J Haematol 111 501–512 Occurrence Handle10.1046/j.1365-2141.2000.02379.x Occurrence Handle1:STN:280:DC%2BD3M7hs1KjsA%3D%3D Occurrence Handle11122091
MN Weitzmann S Cenci L Rifas J Haug J Dipersio R Pacifici (2001) ArticleTitleT cell activation induces human osteoclast formation via receptor activator of nuclear factor ?B ligand-dependent and -independent mechanisms J Bone Miner Res 16 328–337 Occurrence Handle1:CAS:528:DC%2BD3MXptlOqtQ%3D%3D Occurrence Handle11204433
T Shuto T Kukita M Hirata E Jimi T Koga (1994) ArticleTitleDexamethasone stimulates osteoclast-like cell formation by inhibiting granulocyte-macrophage colony-stimulating factor production in mouse bone marrow cultures Endocrinology 134 1121–1126 Occurrence Handle10.1210/en.134.3.1121 Occurrence Handle1:CAS:528:DyaK2cXitVyrsb8%3D Occurrence Handle8119150
H Kaji T Sugimoto M Kanatani K Nishiyama K Chihara (1997) ArticleTitleDexamethasone stimulates osteoclast-like cell formation by directly acting on hemopoietic blast cells and enhances osteoclast-like cell formation stimulated by parathyroid hormone and prostaglandin E2 J Bone Miner Res 12 734–741 Occurrence Handle1:CAS:528:DyaK2sXjtlOqtro%3D Occurrence Handle9144339
LC Hofbauer F Gori BL Riggs DL Lacey CR Dunstan TC Spelsberg S Khosla (1999) ArticleTitleStimulation of osteoprotegerin ligand and inhibition of osteoprotegerin production by glucocorticoids in human osteoblastic lineage cells: potential paracrine mechanisms of glucocorticoid-induced osteoporosis Endocrinology 140 4382–4389 Occurrence Handle10.1210/en.140.10.4382 Occurrence Handle1:CAS:528:DyaK1MXmtFGrurw%3D Occurrence Handle10499489
T Hasegawa Y Yoshimura T Kikuiri Y Yawaka S Takeyama A Matsumoto H Oguchi T Shirakawa (2002) ArticleTitleExpression of receptor activator of NF-kappa B ligand and osteoprotegerin in culture of human periodontal ligament cells J Periodontal Res 37 405–411 Occurrence Handle10.1034/j.1600-0765.2002.01603.x Occurrence Handle1:CAS:528:DC%2BD3sXnsFyjtrc%3D Occurrence Handle12472833
T Hasegawa T Kikuiri S Takeyama Y Yoshimura M Mitome H Oguchi T Shirakawa (2002) ArticleTitleHuman periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro Tissue Cell 34 44–51 Occurrence Handle10.1054/tice.2002.0223 Occurrence Handle1:CAS:528:DC%2BD38XjvFOjs7Y%3D Occurrence Handle11989970
L Huang J Xu SM Kumta MH Zheng (2001) ArticleTitleGene expression of glucocorticoid receptor alpha and beta in giant cell tumour of bone: evidence of glucocorticoid-stimulated osteoclastogenesis by stromal-like tumour cells Mol Cell Endocrinol 181 199–206 Occurrence Handle10.1016/S0303-7207(01)00486-5 Occurrence Handle1:CAS:528:DC%2BD3MXltlKqs74%3D Occurrence Handle11476953
JM Quinn NJ Horwood J Elliott MT Gillespie TJ Martin (2000) ArticleTitleFibroblastic stromal cells express receptor activator of NF-kappa B ligand and support osteoclast differentiation J Bone Miner Res 15 1459–1466 Occurrence Handle1:CAS:528:DC%2BD3cXlvVSjs7Y%3D Occurrence Handle10934644
O Kudo A Sabokbar A Pocock I Itonaga NA Athanasou (2002) ArticleTitleIsolation of human osteoclast formed in vitro: Hormonal effects on bone resorbing activity of human osteoclasts Calcif Tissue Int 71 539–546 Occurrence Handle10.1007/s00223-001-2128-1 Occurrence Handle1:CAS:528:DC%2BD3sXjt1amtg%3D%3D Occurrence Handle12232680
IE James MW Lark D Zembryki EV Lee-Rykaczewski SM Hwang TA Tomaszek P Belfiore M Gowen (1999) ArticleTitleDevelopment and characterization of a human in vitro resorption assay: demonstration of utility using novel antiresorptive agents J Bone Miner Res 14 1562–1569 Occurrence Handle1:CAS:528:DyaK1MXmtVyqurw%3D Occurrence Handle10469285
K Sundquist P Lakkakorpi B Wallmark K Vaananen (1990) ArticleTitleInhibition of osteoclast proton transport by bafilomycin A1 abolishes bone resorption Biochem Biophys Res Commun 168 309–313 Occurrence Handle10.1016/0006-291X(90)91709-2 Occurrence Handle1:CAS:528:DyaK3cXitlarsbc%3D Occurrence Handle2139331
K Suda JT Woo M Takami PM Sexton K Nagai (2002) ArticleTitleLipopolysaccharide supports survival and fusion of preosteoclasts independent of TNF-alpha, IL-1, and RANKL J Cell Physiol 190 101–108 Occurrence Handle10.1002/jcp.10041 Occurrence Handle1:CAS:528:DC%2BD3MXptVKmurg%3D Occurrence Handle11807816
JM Quinn J Elliott MT Gillespie TJ Martin (1998) ArticleTitleA combination of osteoclast differentiation factor and macrophage-colony stimulating factor is sufficient for both human and mouse osteoclast formation in vitro Endocrinology 139 4424–4427 Occurrence Handle10.1210/en.139.10.4424 Occurrence Handle1:CAS:528:DyaK1cXmsVGmsL0%3D Occurrence Handle9751528
M Shiohara AF Gombart Y Sekiguchi E Hidaka S Ito T Yamazaki HP Koeffler A Komiyama (2004) ArticleTitlePhenotypic and functional alterations of peripheral blood monocytes in neutrophil-specific granule deficiency J Leukoc Biol 75 190–197 Occurrence Handle10.1189/jlb.0203063 Occurrence Handle1:CAS:528:DC%2BD2cXhsFKntrY%3D Occurrence Handle14576362
NA Athanasou JI Alvarez FP Ross JM Quinn SL Teitelbaum (1992) ArticleTitleSpecies differences in the immunophenotype of osteoclasts and mononuclear phagocytes Calcif Tissue Int 50 427–432 Occurrence Handle10.1007/BF00296773 Occurrence Handle1:STN:280:By2B1c3ks1Y%3D Occurrence Handle1375864
B Steiniger P Barth B Herbst A Hartnell PR Crocker (1997) ArticleTitleThe species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone Immunology 92 307–316 Occurrence Handle10.1046/j.1365-2567.1997.00328.x Occurrence Handle1:CAS:528:DyaK2sXmsVeltrk%3D Occurrence Handle9415041
HM Massey AM Flanagan (1999) ArticleTitleH. Massey, A. Flanagan, Human osteoclasts derive from CD14-positive monocytes Br J Haematol 106 167–170 Occurrence Handle10.1046/j.1365-2141.1999.01491.x Occurrence Handle1:STN:280:DyaK1MzntlGltw%3D%3D Occurrence Handle10444181
DC Mangham DJ Scoones MT Drayson (1993) ArticleTitleComplement and the recruitment of mononuclear osteoclasts J Clin Pathol 46 517–521 Occurrence Handle1:STN:280:ByyA383ksVM%3D Occurrence Handle8331172
MH Helfrich SA Nesbitt MA Horton (1992) ArticleTitleIntegrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3 J Bone Miner Res 7 345–351 Occurrence Handle1:CAS:528:DyaK38XkvVyhsL0%3D Occurrence Handle1585837
NA Athanasou J Quinn JO McGee (1988) ArticleTitleImmunocytochemical analysis of the human osteoclast: phenotypic relationship to other marrow-derived cells Bone Miner 3 317–333 Occurrence Handle1:STN:280:BiaC2M%2FmtVY%3D Occurrence Handle3228616
A Erices P Conget JJ Minguell (2000) ArticleTitleMesenchymal progenitor cells in human umbilical cord blood Br J Haematol 109 235–242 Occurrence Handle10.1046/j.1365-2141.2000.01986.x Occurrence Handle1:STN:280:DC%2BD3czgvVSqtw%3D%3D Occurrence Handle10848804
L Nagy P Tontonoz JG Alvarez H Chen RM Evans (1998) ArticleTitleOxidized LDL regulates macrophage gene expression through ligand activation of PPARgamma Cell 93 229–240 Occurrence Handle10.1016/S0092-8674(00)81574-3 Occurrence Handle1:CAS:528:DyaK1cXivVygt74%3D Occurrence Handle9568715
B Fernandez Pujol FC Lucibello UM Gehling K Lindemann N Weidner ML Zuzarte J Adamkiewicz HP Elsasser R Muller K Havemann (2000) ArticleTitleEndothelial-like cells derived from human CD14 positive monocytes Differentiation 65 287–300 Occurrence Handle10.1046/j.1432-0436.2000.6550287.x Occurrence Handle1:STN:280:DC%2BD3M%2FivFCnuw%3D%3D Occurrence Handle10929208
J Komi O Lassila (2000) ArticleTitleNonsteroidal anti-estrogens inhibit the functional differentiation of human monocyte-derived dendritic cells Blood 95 2875–2882 Occurrence Handle1:CAS:528:DC%2BD3cXivVaqsr4%3D Occurrence Handle10779434
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Husheem, M., Nyman, J., Vääräniemi, J. et al. Characterization of Circulating Human Osteoclast Progenitors: Development of In Vitro Resorption Assay. Calcif Tissue Int 76, 222–230 (2005). https://doi.org/10.1007/s00223-004-0123-z
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DOI: https://doi.org/10.1007/s00223-004-0123-z