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Cytokine release from adipose tissue of nonobese individuals

International Journal of Obesity volume 29, pages 1144–1147 (2005)Cite this article

Abstract

Explants of human adipose tissue from nonobese subjects were cultured for 24 h with or without the presence of 20 ng/ml TNFα. Gene expression and/or medium concentrations of interleukin (IL)-1β, IL-1 receptor antagonist (IL-1 RA), TNFα, IL-6, IL-8, resistin, PAI-1 and leptin were analysed. TNFα increased the mRNA levels of TNFα itself as well as IL-6, IL-8, IL-1β and PAI-1, but not leptin. The medium concentrations of IL-1 RA, IL-6 and IL-8 were markedly increased by TNFα while no measurable release of TNFα, resistin or IL-1β to the medium was found. Thus, human adipose tissue from nonobese individuals releases substantial amounts of IL-6, IL-8 and IL-1 RA and the gene expression of these cytokines, like that of IL-1β and PAI-1, is regulated by TNFα. However, since neither TNFα, resistin or IL-1β was found in the culture medium, such a regulatory effect by TNFα on adipose tissue in vivo is likely to be mediated through a paracrine mechanism where invaded inflammatory cells may play a critical role.

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Acknowledgements

This study was supported by the grants from the Swedish Research Council (K2004-72X-03506-33A), the Torsten and Ragnar Söderbergs Foundation, the European Community (QLG1-1999-CT-00674), the Swedish Diabetes Association, the Sonya Hedenbratt Memorial Fund and the IngaBritt and Arne Lundberg Foundation.

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  1. Department of Internal Medicine, The Lundberg Laboratory for Diabetes Research, The Sahlgrenska Academy at Göteborg University, Göteborg, Sweden

    V R Sopasakis, I Nagaev & U Smith

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  1. V R Sopasakis
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  2. I Nagaev
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Correspondence to V R Sopasakis.

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Sopasakis, V., Nagaev, I. & Smith, U. Cytokine release from adipose tissue of nonobese individuals. Int J Obes 29, 1144–1147 (2005). https://doi.org/10.1038/sj.ijo.0803002

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