TY - JOUR T1 - Role of synovial fibroblast subsets across synovial pathotypes in rheumatoid arthritis: a deconvolution analysis JF - RMD Open JO - RMD Open DO - 10.1136/rmdopen-2021-001949 VL - 8 IS - 1 SP - e001949 AU - Raphael Micheroli AU - Muriel Elhai AU - Sam Edalat AU - Mojca Frank-Bertoncelj AU - Kristina Bürki AU - Adrian Ciurea AU - Lucy MacDonald AU - Mariola Kurowska-Stolarska AU - Myles J Lewis AU - Katriona Goldmann AU - Cankut Cubuk AU - Tadeja Kuret AU - Oliver Distler AU - Costantino Pitzalis AU - Caroline Ospelt Y1 - 2022/01/01 UR - http://rmdopen.bmj.com/content/8/1/e001949.abstract N2 - Objectives To integrate published single-cell RNA sequencing (scRNA-seq) data and assess the contribution of synovial fibroblast (SF) subsets to synovial pathotypes and respective clinical characteristics in treatment-naïve early arthritis.Methods In this in silico study, we integrated scRNA-seq data from published studies with additional unpublished in-house data. Standard Seurat, Harmony and Liger workflow was performed for integration and differential gene expression analysis. We estimated single cell type proportions in bulk RNA-seq data (deconvolution) from synovial tissue from 87 treatment-naïve early arthritis patients in the Pathobiology of Early Arthritis Cohort using MuSiC. SF proportions across synovial pathotypes (fibroid, lymphoid and myeloid) and relationship of disease activity measurements across different synovial pathotypes were assessed.Results We identified four SF clusters with respective marker genes: PRG4+ SF (CD55, MMP3, PRG4, THY1neg); CXCL12+ SF (CXCL12, CCL2, ADAMTS1, THY1low); POSTN+ SF (POSTN, collagen genes, THY1); CXCL14+ SF (CXCL14, C3, CD34, ASPN, THY1) that correspond to lining (PRG4+ SF) and sublining (CXCL12+ SF, POSTN+ + and CXCL14+ SF) SF subsets. CXCL12+ SF and POSTN+ + were most prominent in the fibroid while PRG4+ SF appeared highest in the myeloid pathotype. Corresponding, lining assessed by histology (assessed by Krenn-Score) was thicker in the myeloid, but also in the lymphoid pathotype + the fibroid pathotype. PRG4+ SF correlated positively with disease severity parameters in the fibroid, POSTN+ SF in the lymphoid pathotype whereas CXCL14+ SF showed negative association with disease severity in all pathotypes.Conclusion This study shows a so far unexplored association between distinct synovial pathologies and SF subtypes defined by scRNA-seq. The knowledge of the diverse interplay of SF with immune cells will advance opportunities for tailored targeted treatments.Data are available in a public, open access repository. All data relevant to the study are included in the article or uploaded as online supplemental information. All data relevant to the study are included in the article, uploaded as online supplemental information or already publicly available. ER -