Abstract
The acute effects of mineralo- and glucocorticoids on urinary electrolyte excretion were studied in the conscious, acutely potassium deprived, adrenalectomized rat. Sodium, potassium, and creatinine were measured in the urine excreted from 2.5 to 5.5 h after injection of one or more of the following steroids: aldosterone (Aldo), 9-alpha fluorocortisol (FC), deoxycorticosterone (DOC), dexamethasone (Dex), and spironolactone (Spiro). The hierarchy (a) for increasing creatinine excretion was Dex>FC>Aldo>DOC>Spiro >none, a hierarchy consistent with glucocorticoid potency; and (b) for producing anti-natriuresis was Aldo>DOC≥FC ≥none=Spiro>Dex, a hierarchy consistent with mineralocorticoid potency. In contrast, the kaliuresis produced by mineralo- and glucocorticoids appears different. A “mineralocorticoid” kaliuresis is 1) elicited by anti-natriuretic doses of Aldo and FC, 2) approximately twice control UKV, 3) unrelated to changes in glomerular filtration rate (GFR), and 4) inhibited by Spiro. A “glucocorticoid” kaliuresis is 1) elicited by Dex and high doses of Aldo and FC, 2) about seven to twenty-fold greater than control UKV, 3) possibly dependent, in part, on changes in GFR, and, 4) not inhibited by Spiro. DOC was not kaliuretic at anti-natriuretic doses. The urinary Na/K ratio was an unreliable index of mineralocorticoid action.
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References
Bailey RE (1960) Acute effects of corticosteroid mixtures and the 17-spirolactone SC-8109 on sodium, potassium, and water excretion in adrenalectomized, water-loaded rats. Endocrinology 67:807–814
Barger AC, Berlin RD, Tulenko JF (1958) Infusion of aldosterone, 9α-fluorohydrocortisone and antidiuretic hormone into the renal artery of normal and adrenalectomized, unanesthetized dogs: effect on electrolyte and water excretion. Endocrinology 62:804–815
Bia MJ, Tyler K, DeFronzo RA (1982) The effect of dexamethasone on renal electrolyte excretion in the adrenalectomized rat. Endocrinology 111:882–888
Campen TJ, Fanestil DD (1982) Spironolactone: a glucocorticoid agonist or antagonist? Clin Exp Hypertens 4:1627–1636
Fanestil DD, Park CS (1981) Steroid hormones and the kidney. Ann Rev Physiol 43:637–649
Feldman D, Funder JW, Edelman IS (1972) Subcellular mechanisms in the action of adrenal steroids. Am J Med 53:545–560
Fimognari GM, Fanestil DD, Edelman IS (1967) Induction of RNA and protein synthesis in the action of aldosterone in the rat. Am J Physiol 213:954–962
Folin O (1914) On the determination of creatinine and creatine in urine. J Biol Chem 17:469–473
Folin O, Wu H (1919) A system of blood analysis. J Biol Chem 38:81–110
Good DW, Wright FS (1979) Luminal influences on potassium secretion: sodium concentration and fluid flow rate. Am J Physiol 236:F192-F205
Hiatt N, Chapman LW, Davidson MB, Sheinkopf JA (1979) Adrenal hormones and the regulation of serum potassium in potassiumloaded adrenalectomized dogs. Endocrinology 105:215–219
Hierholzer K, Wiederholt M, Holzgreve H, giebisch G, Klose RM, Windhager EE (1965) Micropuncture study of renal transtubular concentration gradients of sodium and potassium in adrenalectomized rats. Pflügers Arch 285:193–210
Hulter HN, Bonner EL Jr, Glynn RD, Sebastian A (1981) Renal and systemic acid-base effects of chronic spironolactone administration. Am J Physiol 240: (Renal Fluid Electrolyte Physiol 9) F381-F387
Malnic G, Klose RM, Giebisch G (1966) Micropuncture study of distal tubular potassium and sodium transport in rat nephron. Am J Physiol 211:529–547
Rabinowitz L (1979/80) Aldosterone and renal potassium excretion. Renal Physiol 2:229–243
Raynaud JP, Bouton MM, Ojasoo T (1979) Design of anti-hormones. Biochem Soc Trans 7:547–551
Rodriguez HJ, Sinha SK, Starling J, Klahr S (1981) Regulation of Na+−K+−ATPase in the rat by adrenal steroids. Am J Physiol 241 (Renal Fluid Electrolyte Physiol 10):F186-F195
Ryan TA Jr, Joiner BL, Ryan BF (1976) Nonparametric statistics. In: Minitab student handbook. Chapter 12. Duxbury Press, North Scituate, Massachusetts, p 258
Samuels HH, Tomkins GM (1970) Relation of steroid structure to enzyme induction in hepatoma tissue culture cells. J Mol Biol 52:57–74
Schwartz GJ, Burg MB (1978) Mineralocorticoid effects on cation transport by cortical collecting tubules in vitro. Am J Physiol 235(6):F576-F585
Schwartz WB, Jensen RL, Relman AS (1955) Acidification of the urine and increased ammonium excretion without change in acid-base equilibrium: sodium reabsorption as a stimulus to the acidifying process. J Clin Invest 34:673–680
Sinha SK, Rodriguez HJ, Hogan WC, Klahr S (1981) Mechanisms of activation of renal (Na++K+)-ATPase in the rat. Effects of acute and chronic administration of dexamethasone. Biochim Biophys Acta 641:20–35
Snedecor GW, Cochran WG (1980) Shortcut and nonparametric methods. In: Statistical methods. Chapter 8. The Iowa State University Press, Ames, Iowa, USA, p 144
Uete T, Venning EH (1962) Interplay between various adrenal cortical steroids with respect to electrolyte excretion. Endocrinology 71:768–778
Vander AJ, Malvin RL, Wilde WS, Lapides J, Sullivan LP, McMurray VM (1958) Effects of adrenalectomy and aldosterone on proximal and distal tubular sodium reabsorption. Proc Soc Exp Biol Med 99:323–325
Van Slyke DD, Hawkins JA (1928) A gasometric method for determination of reducing sugars and its application to analysis of blood and urine. J Biol Chem 79:739–767
Wiederholt M, Behn C, Schoormans W, Hansen L (1972) Effect of aldosterone on sodium and potassium transport in the kidney. J Steroid Biochem 3:151–159
Wilcox CS, Cemerick DA, Giebisch G (1982) Differential effects of acute mineralo- and glucocorticoid administration on renal acid elimination. Kidney Int 21:546–556
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Campen, T.J., Vaughn, D.A. & Fanestil, D.D. Mineralo- and glucocorticoid effects on renal excretion of electrolytes. Pflugers Arch. 399, 93–101 (1983). https://doi.org/10.1007/BF00663903
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DOI: https://doi.org/10.1007/BF00663903