Bone marrow/stem cell transplantationEotaxin/CCL11 Levels Correlate With Myocardial Fibrosis and Mast Cell Density in Native and Transplanted Rat Hearts
Section snippets
Materials and Methods
For this study we used paraformaldhyde-fixed and paraffin-embedded rat heart tissues from previous work.15, 23 To determine myocardial fibrosis, collagen fibrils were stained with Masson's trichrome reagent. For co-localization of RMCP-1 and -2, we performed immunofluorescence double staining using a polyclonal sheep antibody to RMCP-1 (0.625 μg/mL) and a monoclonal mouse antibody to RMCP-2 (1.25 μg/mL; Moredun Scientific Ltd., Midlothian, Scotland, United Kingdom) followed by a mixture of
Results
The area of myocardial fibrosis was significantly increased in the allogeneic compared with the isogeneic group on day 16 (38% vs 21%) and on day 28 (49% vs 22%) after transplantation (Fig 1A). The isogeneic transplants showed increased fibrosis compared with native hearts (4%), which remained constant (between 22% and 24%) over all time points. All hearts were beating at the time of harvest.
A significant correlation was observed between the levels of myocardial fibrosis and eotaxin/CCL11
Discussion
Compared with isografts at 28 days after transplantation, myocardial fibrosis significantly increases in rat heart allografts during ongoing acute rejection. This change correlates with eotaxin/CCL11 concentrations and the density of MMC, but not CTMC in heart tissue. Myocardial fibrosis and histological changes in the transplanted heart, as well described in long-term surviving patients, are likely to affect short-term and long-term cardiac function. The fibrotic response occurs as early as 1
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2019, European Journal of PharmacologyCitation Excerpt :It has been reported that cardiac macrophages produced eotaxin during heart transplant rejection, which attract MCs into myocardial. MC mediated myocardial fibrosis correlates remarkably with CCL11 concentrations and the density of myocardial MC in heart tissue (Zweifel et al., 2010). Repopulation of vein grafts with infiltrating MCs occurs rapidly after grafting.
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2017, Clinical NutritionCitation Excerpt :Eotaxin expression levels increase with adipocyte differentiation [25], whereas its levels decrease in visceral adipose tissues when HFD-fed mice undergo weight loss [25]. In addition, eotaxin participates in cardiac fibrogenesis by triggering mast cell infiltration of the heart [24]. The O-MHO pigs exhibited a higher plasma level of eotaxin than C-MHO pigs, suggesting its potential role in the development of cardiac fibrosis.
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2015, Advances in ImmunologyCitation Excerpt :The eotaxin has a profibrogenic effect on human lung FBs (Kohan, Puxeddu, Reich, Levi-Schaffer, & Berkman, 2010; Puxeddu et al., 2006). Therefore, targeting eotaxin with mAb, such as Bertilimumab, could reduce MCs infiltration and associated fibrosis (Mangieri et al., 2012; Zweifel, Matozan, Dahinden, Schaffner, & Mohacsi, 2010). Another selective and competitive CCR3 antagonist NCT01160224 is under evaluation in phase II trials for its effectiveness in reducing sputum eosinophilia in mild to moderate asthma.
This work was supported by the Katharina Huber-Steiner Foundation.