Abstract
The myeloperoxidase (MPO) system of activated phagocytes is central to normal host defense mechanisms, and dysregulated MPO contributes to the pathogenesis of inflammatory disease states ranging from atherosclerosis to cancer. Here we show that upon systemic administration, the small molecule luminol enables noninvasive bioluminescence imaging (BLI) of MPO activity in vivo. Luminol-BLI allowed quantitative longitudinal monitoring of MPO activity in animal models of acute dermatitis, mixed allergic contact hypersensitivity, focal arthritis and spontaneous large granular lymphocytic tumors. Bioluminescence colocalized with histological sites of inflammation and was totally abolished in gene-deleted Mpo−/− mice, despite massive tissue infiltration of neutrophils and activated eosinophils, indicating that eosinophil peroxidase did not contribute to luminol-BLI in vivo. Thus, luminol-BLI provides a noninvasive, specific and highly sensitive optical readout of phagocyte-mediated MPO activity in vivo and may enable new diagnostic applications in a wide range of acute and chronic inflammatory conditions.
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Acknowledgements
We thank V. Sharma for insightful discussions. This research was funded by US National Institutes of Health grants P50 CA94056, CA105218, CA10073, CA63417 and PO1 HL030086.
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S.G. designed and performed most of the experiments, spearheaded data analysis and wrote and edited the manuscript; S.T.G. performed experiments and edited the manuscript; B.L.M. performed experiments and edited the manuscript; D.R. assisted with experiments and edited the manuscript; J.H. assisted with experiments; J.W.H. provided crucial reagents, helped design experiments and edited the manuscript; L.R. provided crucial reagents, helped design experiments and edited the manuscript; D.P.-W. guided the project, designed experiments, analyzed data and wrote and edited the manuscript.
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Gross, S., Gammon, S., Moss, B. et al. Bioluminescence imaging of myeloperoxidase activity in vivo. Nat Med 15, 455–461 (2009). https://doi.org/10.1038/nm.1886
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DOI: https://doi.org/10.1038/nm.1886
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