Abstract
We have studied the effect of intravenous immunoglobulins (IVIG) on monocyte subpopulations and cytokine production in patients with CVID. The absolute number of CD14(+)CD16(++) monocytes decreased on average 2.5-fold 4h after IVIG and after 20h returned to the baseline. The cytokine level in the supernatants of peripheral blood mononuclear cells (PBMC) after ex vivo LPS stimulation demonstrated the >2-fold decrease in TNF production 4h after IVIG. The TNF expression, which is higher in the CD14(+)CD16(++) monocytes, was decreased in these cells by IVIG in 4/7 CVID cases. In vitro exposure of the healthy individuals' monocytes to the IVIG preparation resulted in reduced TNF production, which was overcome by blockade of the FcγRIIB in the CD14(+)CD16(++) CD32B(high) monocytes. Our data suggest that reduction in the number of CD14(+)CD16(++) monocytes and the blockade of their cytokine production via triggering CD32B can contribute to the anti-inflammatory action of IVIG.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
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Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antibodies, Monoclonal / pharmacology
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Antigens, CD / metabolism
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Common Variable Immunodeficiency / therapy*
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Female
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GPI-Linked Proteins / metabolism
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HLA-DR Antigens / metabolism
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Humans
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Immunoglobulins, Intravenous / pharmacology*
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Immunoglobulins, Intravenous / therapeutic use
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Immunophenotyping
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Interleukin-10 / metabolism
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Interleukin-12 / metabolism
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Kinetics
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Leukocyte Count
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Leukocytes, Mononuclear / drug effects
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Leukocytes, Mononuclear / metabolism
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Lipopolysaccharide Receptors / metabolism*
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Lipopolysaccharides / pharmacology
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Male
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Middle Aged
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Monocytes / cytology
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Monocytes / drug effects*
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Monocytes / immunology
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Monocytes / metabolism
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Receptors, IgG / immunology
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Receptors, IgG / metabolism*
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Tumor Necrosis Factor-alpha / metabolism
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Young Adult
Substances
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Antibodies, Monoclonal
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Antigens, CD
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FCGR3B protein, human
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Fc gamma receptor IIB
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GPI-Linked Proteins
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HLA-DR Antigens
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IL10 protein, human
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Immunoglobulins, Intravenous
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Lipopolysaccharide Receptors
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Lipopolysaccharides
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Receptors, IgG
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Interleukin-12