Abstract
Herpesvirus Saimiri gene 13 (HVS13) exhibits 57% identity with the predicted sequence of a T cell-derived molecule termed CTLA8. Recombinant HVS13 and CTLA8 stimulate transcriptional factor NF-kappa B activity and interleukin-6 (IL-6) secretion in fibroblasts, and costimulate T cell proliferation. An HVS13.Fc fusion protein was used to isolate a cDNA encoding a novel receptor that also binds CTLA8. This receptor is unrelated to previously identified cytokine receptor families. A recombinant soluble receptor inhibited T cell proliferation and IL-2 production induced by PHA, concanavalin A (conA), and anti-TCR MAb. These results define CTLA8 and HVS13 as novel cytokines that bind to a novel cytokine receptor. We propose to call these molecules IL-17, vIL-17, and IL-17R, respectively.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Viral / immunology
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Antigens, Viral / isolation & purification
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Antigens, Viral / pharmacology
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Base Sequence
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Cell Division / drug effects
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Cell Line
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Cloning, Molecular
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Herpesvirus 2, Saimiriine / genetics
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Herpesvirus 2, Saimiriine / immunology*
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Interleukin-17
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Interleukins / immunology
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Interleukins / isolation & purification*
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Interleukins / pharmacology
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Mice
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Molecular Sequence Data
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Receptors, Cytokine / immunology
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Receptors, Cytokine / isolation & purification*
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Receptors, Immunologic / immunology
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Receptors, Immunologic / isolation & purification*
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Recombinant Fusion Proteins / immunology
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T-Lymphocytes / immunology*
Substances
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Antigens, Viral
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Interleukin-17
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Interleukins
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Receptors, Cytokine
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Receptors, Immunologic
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Recombinant Fusion Proteins